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Crystallization of the pneumococcal autolysin LytC: in-house phasing using novel lanthanide complexes

LytC, one of the major autolysins from the human pathogen Streptococcus pneumoniae, has been crystallized as needles by the hanging-drop technique using 10%(w/v) PEG 3350 as precipitant and 10 mM HEPES pH 7.5. LytC crystals were quickly soaked in mother liquor containing 2 mM of the complex Gd-HPDO3...

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Detalhes bibliográficos
Main Authors: Pérez-Dorado, Inmaculada, Sanles, Reyes, González, Ana, García, Pedro, García, José L., Martínez-Ripoll, Martín, Hermoso, Juan A.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2852341/
https://ncbi.nlm.nih.gov/pubmed/20383019
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110006081
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