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New Vector System for Random, Single-Step Integration of Multiple Copies of DNA into the Rhodococcus Genome
We designed a new vector system for creating a random mutant library with multiple integrations of DNA fragments into the Rhodococcus genome in a single step. For this, we cotransformed two vectors into Rhodococcus by electroporation: pTip-istAB-sacB regulates the expression of the transposase (IstA...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849190/ https://ncbi.nlm.nih.gov/pubmed/20154109 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02131-09 |
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