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RAPID RELEASE OF N-LINKED GLYCANS FROM GLYCOPROTEINS BY PRESSURE CYCLING TECHNOLOGY
The standard, well-established sample preparation protocol to release N-linked glycans from glycoproteins for downstream analysis requires relatively long deglycosylation times (from several hours to overnight) and relatively high endoglycosidase concentration (1:250 – 1:500 enzyme:substrate molar r...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2010
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2847834/ https://ncbi.nlm.nih.gov/pubmed/20170179 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac100098e |
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