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RAPID RELEASE OF N-LINKED GLYCANS FROM GLYCOPROTEINS BY PRESSURE CYCLING TECHNOLOGY

The standard, well-established sample preparation protocol to release N-linked glycans from glycoproteins for downstream analysis requires relatively long deglycosylation times (from several hours to overnight) and relatively high endoglycosidase concentration (1:250 – 1:500 enzyme:substrate molar r...

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Autores principales: Szabo, Zoltan, Guttman, András, Karger, Barry L.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847834/
https://ncbi.nlm.nih.gov/pubmed/20170179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac100098e
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