Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli

Despite recent advances in sequencing, complete finishing of large genomes and analysis of novel proteins they encode typically require cloning of specific regions. However, many of these fragments are extremely difficult to clone in current vectors. Superhelical stress in circular plasmids can gene...

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Bibliographische Detailangaben
Hauptverfasser: Godiska, Ronald, Mead, David, Dhodda, Vinay, Wu, Chengcang, Hochstein, Rebecca, Karsi, Attila, Usdin, Karen, Entezam, Ali, Ravin, Nikolai
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2010
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847241/
https://ncbi.nlm.nih.gov/pubmed/20040575
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1181
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