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Linear plasmid vector for cloning of repetitive or unstable sequences in Escherichia coli
Despite recent advances in sequencing, complete finishing of large genomes and analysis of novel proteins they encode typically require cloning of specific regions. However, many of these fragments are extremely difficult to clone in current vectors. Superhelical stress in circular plasmids can gene...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2847241/ https://ncbi.nlm.nih.gov/pubmed/20040575 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1181 |
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