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One-plasmid tunable coexpression for mycobacterial protein–protein interaction studies
A single plasmid that allows controlled coexpression has been developed for use in mycobacteria. The tetracycline inducible promoter, PtetO, was used to provide tetracycline-dependent induction of one gene, while the Psmyc, Pimyc, or Phsp promoters were used to provide three different levels of cons...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2788286/ https://ncbi.nlm.nih.gov/pubmed/19760663 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.242 |
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