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Differential binding of Escherichia coli McrA protein to DNA sequences that contain the dinucleotide m5CpG

The Escherichia coli McrA protein, a putative C(5)-methylcytosine/C(5)-hydroxyl methylcytosine-specific nuclease, binds DNA with symmetrically methylated HpaII sequences (Cm5CGG), but its precise recognition sequence remains undefined. To determine McrA’s binding specificity, we cloned and expressed...

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Bibliografske podrobnosti
Main Authors: Mulligan, Elizabeth A., Hatchwell, Eli, McCorkle, Sean R., Dunn, John J.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847215/
https://ncbi.nlm.nih.gov/pubmed/20015968
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp1120
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