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Cloning, purification and initial characterization of E. coli McrA, a putative 5-methylcytosine-specific nuclease

Expression strains of Escherichia coli BL21(DE3) overproducing the E. coli m(5)C McrA restriction protein were produced by cloning the mcrA coding sequence behind a T7 promoter. The recombinant mcrA minus BL21 (DE3) host produces active McrA as evidenced by its acquired ability to selectively restri...

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Detalhes bibliográficos
Main Authors: Mulligan, Elizabeth A., Dunn, John J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2900843/
https://ncbi.nlm.nih.gov/pubmed/18662788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.06.016
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