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High-resolution temperature-concentration diagram of α-synuclein conformation obtained from a single Förster resonance energy transfer image in a microfluidic device

We present a microfluidic device for rapid and efficient determination of protein conformations in a range of medium conditions and temperatures. The device generates orthogonal gradients of concentration and temperature in an interrogation area that fits into the field of view of an objective lens...

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Detalhes bibliográficos
Main Authors: Vandelinder, Virginia, Ferreon, Allan Chris M., Gambin, Yann, Deniz, Ashok A., Groisman, Alex
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2846235/
https://ncbi.nlm.nih.gov/pubmed/19555081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac901008c
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