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Rapid Inactivation of Proteins by Rapamycin-Induced Rerouting to Mitochondria

We have developed a method for rapidly inactivating proteins with rapamycin-induced heterodimerization. Cells were stably transfected with siRNA-resistant, FKBP-tagged subunits of the adaptor protein (AP) complexes of clathrin-coated vesicles (CCVs), together with an FKBP and rapamycin-binding domai...

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Bibliografiske detaljer
Main Authors: Robinson, Margaret S., Sahlender, Daniela A., Foster, Samuel D.
Format: Artigo
Sprog:Inglês
Udgivet: Cell Press 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2845799/
https://ncbi.nlm.nih.gov/pubmed/20159602
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2009.12.015
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