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Rapid Inactivation of Proteins by Rapamycin-Induced Rerouting to Mitochondria
We have developed a method for rapidly inactivating proteins with rapamycin-induced heterodimerization. Cells were stably transfected with siRNA-resistant, FKBP-tagged subunits of the adaptor protein (AP) complexes of clathrin-coated vesicles (CCVs), together with an FKBP and rapamycin-binding domai...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Cell Press
2010
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2845799/ https://ncbi.nlm.nih.gov/pubmed/20159602 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2009.12.015 |
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