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Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast
Genetic analysis of protein function requires a rapid means of inactivating the gene under study. Typically this exploits temperature sensitive mutations, or promoter shut-off techniques. We report the adaptation to Schizosaccharomyces pombe of the Anchor Away technique, originally designed in buddi...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4106978/ https://ncbi.nlm.nih.gov/pubmed/24733494 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/yea.3014 |
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