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K depletion increases protein tyrosine kinase-mediated phosphorylation of ROMK

We purified Histagged ROMK1 and carried out in vitro phosphorylation assays with (32)P-radiolabeled ATP to determine whether ROMK1 protein is a substrate for PTK. Addition of active c-Src and [(32)P]ATP to the purified ROMK1 protein resulted in the phosphorylation of the ROMK1 protein. However, c-Sr...

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Dettagli Bibliografici
Autori principali: Lin, Dao-Hong, Sterling, Hyacinth, Lerea, Kenneth M., Welling, Paul, Jin, Lianhong, Giebisch, Gerhard, Wang, Wen-Hui
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2843414/
https://ncbi.nlm.nih.gov/pubmed/12217858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajprenal.00160.2002
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