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How Escherichia coli can bias the results of molecular cloning: Preferential selection of defective genomes of hepatitis C virus during the cloning procedure

Cloned PCR products containing hepatitis C virus (HCV) genomic fragments have been used for analyses of HCV genomic heterogeneity and protein expression. These studies assume that the clones derived are representative of the entire virus population and that subsets are not inadvertently selected. Th...

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書誌詳細
主要な著者: Forns, Xavier, Bukh, Jens, Purcell, Robert H., Emerson, Suzanne U.
フォーマット: Artigo
言語:Inglês
出版事項: National Academy of Sciences 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC28406/
https://ncbi.nlm.nih.gov/pubmed/9391126
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