How Escherichia coli can bias the results of molecular cloning: Preferential selection of defective genomes of hepatitis C virus during the cloning procedure

Cloned PCR products containing hepatitis C virus (HCV) genomic fragments have been used for analyses of HCV genomic heterogeneity and protein expression. These studies assume that the clones derived are representative of the entire virus population and that subsets are not inadvertently selected. Th...

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Autors principals: Forns, Xavier, Bukh, Jens, Purcell, Robert H., Emerson, Suzanne U.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1997
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC28406/
https://ncbi.nlm.nih.gov/pubmed/9391126
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