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Induced allostery in the directed evolution of an enantioselective Baeyer–Villiger monooxygenase

The molecular basis of allosteric effects, known to be caused by an effector docking to an enzyme at a site distal from the binding pocket, has been studied recently by applying directed evolution. Here, we utilize laboratory evolution in a different way, namely to induce allostery by introducing ap...

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Autori principali: Wu, Sheng, Acevedo, Juan Pablo, Reetz, Manfred T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2840326/
https://ncbi.nlm.nih.gov/pubmed/20133612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0911656107
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