Sensitive in vivo imaging of T cells utilizing a membrane bound Gaussia princeps luciferase enzyme

We developed a novel approach to bioluminescent T cell imaging (BLI) using a membrane-anchored form of the Gaussia luciferase (GLuc) enzyme, termed extGLuc, which we could stably express in both mouse and human primary T cells. In vitro, extGLuc(+) cells emitted significantly higher bioluminescent s...

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Detalhes bibliográficos
Main Authors: Santos, Elmer B, Yeh, Raymond, Lee, James, Nikhamin, Yan, Punzalan, Blesida, Punzalan, Bleserene, La Perle, Krista, Larson, Steven M, Sadelain, Michel, Brentjens, Renier J
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2837150/
https://ncbi.nlm.nih.gov/pubmed/19219023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nm.1930
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