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Highly Avid Magnetic Bead Capture: An Efficient Selection Method for de novo Protein Engineering Utilizing Yeast Surface Display

Protein engineering relies on the selective capture of members of a protein library with desired properties. Yeast surface display technology routinely enables as much as million-fold improvements in binding affinity by alternating rounds of diversification and flow cytometry-based selection. Howeve...

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Hlavní autoři: Ackerman, Margaret, Levary, David, Tobon, Gabriel, Hackel, Benjamin, Davis Orcutt, Kelly, Wittrup, K. Dane
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2837102/
https://ncbi.nlm.nih.gov/pubmed/19363813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/btpr.174
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