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Nanopore-based sequence-specific detection of duplex DNA for genomic profiling

We demonstrate a purely electrical method for single-molecule detection of specific DNA sequences, achieved by hybridizing double-stranded DNA (dsDNA) with peptide nucleic acid (PNA) probes and electrophoretically threading the DNA through sub-5 nm silicon nitride pores. Bis-PNAs were used as the ta...

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Detalhes bibliográficos
Main Authors: Singer, Alon, Wanunu, Meni, Morrison, Will, Kuhn, Heiko, Frank-Kamenetskii, Maxim, Meller, Amit
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2834191/
https://ncbi.nlm.nih.gov/pubmed/20088590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl100058y
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