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The value of using multiple proteases for large-scale mass spectrometry-based proteomics

Large-scale protein sequencing methods rely on enzymatic digestion of complex protein mixtures to generate a collection of peptides for mass spectrometric analysis. Here we examine the use of multiple proteases (trypsin, LysC, ArgC, AspN, and GluC) to improve both protein identification and characte...

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Bibliografski detalji
Glavni autori: Swaney, Danielle L., Wenger, Craig D., Coon, Joshua J.
Format: Artigo
Jezik:Inglês
Izdano: 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2833215/
https://ncbi.nlm.nih.gov/pubmed/20113005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr900863u
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