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The value of using multiple proteases for large-scale mass spectrometry-based proteomics
Large-scale protein sequencing methods rely on enzymatic digestion of complex protein mixtures to generate a collection of peptides for mass spectrometric analysis. Here we examine the use of multiple proteases (trypsin, LysC, ArgC, AspN, and GluC) to improve both protein identification and characte...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2833215/ https://ncbi.nlm.nih.gov/pubmed/20113005 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr900863u |
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