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In Vitro Biosynthesis of Pseudouridine at the Polynucleotide Level by an Enzyme Extract from Escherichia coli

DNA from Mycoplasma sp. Kid which was enriched for tRNA genes (containing about 10% tDNA) was transcribed by E. coli RNA polymerase. The RNA transcription product labeled with [(14)C]uridine was formed in good yield (70-fold net synthesis). After incubation of this [(14)C]uridine-labeled RNA with E....

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Detalhes bibliográficos
Main Authors: Johnson, Lee, Söll, Dieter
Formato: Artigo
Idioma:Inglês
Publicado em: 1970
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC283296/
https://ncbi.nlm.nih.gov/pubmed/4943184
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