In Vitro Biosynthesis of Pseudouridine at the Polynucleotide Level by an Enzyme Extract from Escherichia coli
DNA from Mycoplasma sp. Kid which was enriched for tRNA genes (containing about 10% tDNA) was transcribed by E. coli RNA polymerase. The RNA transcription product labeled with [(14)C]uridine was formed in good yield (70-fold net synthesis). After incubation of this [(14)C]uridine-labeled RNA with E....
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
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| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1970
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC283296/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4943184/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.67.2.943 |
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