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In Vitro Biosynthesis of Pseudouridine at the Polynucleotide Level by an Enzyme Extract from Escherichia coli
DNA from Mycoplasma sp. Kid which was enriched for tRNA genes (containing about 10% tDNA) was transcribed by E. coli RNA polymerase. The RNA transcription product labeled with [(14)C]uridine was formed in good yield (70-fold net synthesis). After incubation of this [(14)C]uridine-labeled RNA with E....
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1970
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC283296/ https://ncbi.nlm.nih.gov/pubmed/4943184 |
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