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A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity

Currently, there is no reliable system for regulated gene expression and regulated gene knockdown in cells with finite lifespan. In this manuscript, we describe a vector system, consisting of a retrovirus for the delivery of rtTA, and a lentivirus for the delivery of either a transgene or a miR-shRN...

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Autori principali: Alexeyev, Mikhail F., Fayzulin, Rafik, Shokolenko, Inna N., Pastukh, Viktoriya
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2009
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2832089/
https://ncbi.nlm.nih.gov/pubmed/19655272
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11033-009-9647-7
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