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A retro-lentiviral system for doxycycline-inducible gene expression and gene knockdown in cells with limited proliferative capacity
Currently, there is no reliable system for regulated gene expression and regulated gene knockdown in cells with finite lifespan. In this manuscript, we describe a vector system, consisting of a retrovirus for the delivery of rtTA, and a lentivirus for the delivery of either a transgene or a miR-shRN...
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| Asıl Yazarlar: | , , , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2009
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2832089/ https://ncbi.nlm.nih.gov/pubmed/19655272 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11033-009-9647-7 |
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