A Robust Two-Dimensional Separation for Top-Down Tandem Mass Spectrometry of the Low-Mass Proteome

For fractionation of intact proteins by molecular weight (MW), a sharply improved two-dimensional (2D) separation is presented to drive reproducible and robust fractionation before top-down mass spectrometry of complex mixtures. The “GELFrEE” (i.e., gel-eluted liquid fraction entrapment electrophore...

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Detalhes bibliográficos
Main Authors: Lee, Ji Eun, Kellie, John F., Tran, John C., Tipton, Jeremiah D., Catherman, Adam D., Thomas, Haylee M., Ahlf, Dorothy R., Durbin, Kenneth R., Vellaichamy, Adaikkalam, Ntai, Ioanna, Marshall, Alan G., Kelleher, Neil L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2830800/
https://ncbi.nlm.nih.gov/pubmed/19747844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jasms.2009.08.001
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