Purification and reconstitution of the proton-translocating ATPase of Golgi-enriched membranes.

Kidney cortex microsomes enriched in Golgi markers and probably also containing endosomes were isolated by cell fractionation and found to contain a proton-translocating ATPase that was inhibited by N-ethylmaleimide (NEM). This NEM-sensitive ATPase was solubilized with n-octyl glucoside and purified...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Young, G P, Qiao, J Z, Al-Awqati, Q
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1988
Pynciau:
Research Article
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282806/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2904677/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.24.9590
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