Purification and reconstitution of the proton-translocating ATPase of Golgi-enriched membranes.

Kidney cortex microsomes enriched in Golgi markers and probably also containing endosomes were isolated by cell fractionation and found to contain a proton-translocating ATPase that was inhibited by N-ethylmaleimide (NEM). This NEM-sensitive ATPase was solubilized with n-octyl glucoside and purified...

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Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Young, G P, Qiao, J Z, Al-Awqati, Q
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 1988
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282806/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2904677/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.24.9590
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