Purification and reconstitution of the proton-translocating ATPase of Golgi-enriched membranes.
Kidney cortex microsomes enriched in Golgi markers and probably also containing endosomes were isolated by cell fractionation and found to contain a proton-translocating ATPase that was inhibited by N-ethylmaleimide (NEM). This NEM-sensitive ATPase was solubilized with n-octyl glucoside and purified...
Spremljeno u:
| Izdano u: | Proc Natl Acad Sci U S A |
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| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1988
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282806/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2904677/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.24.9590 |
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