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Metal and substrate binding to Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis
The addition of divalent metal ions or substrate taurine to TauD, an α-ketoglutarate dependent dioxygenase, alters its UV absorption, as clearly observed by monitoring the protein’s difference spectra. Binding of metal ions leads to a decrease in absorption at ~297 nm and modulation of other feature...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2823959/ https://ncbi.nlm.nih.gov/pubmed/19932076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.11.022 |
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