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Thermodynamic and Kinetic Framework of Selenocysteyl-tRNA(Sec) Recognition by Elongation Factor SelB
SelB is a specialized translation elongation factor that delivers selenocysteyl-tRNA(Sec) (Sec-tRNA(Sec)) to the ribosome. Here we show that Sec-tRNA(Sec) binds to SelB·GTP with an extraordinary high affinity (K(d) = 0.2 pm). The tight binding is driven enthalpically and involves the net formation o...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2823455/ https://ncbi.nlm.nih.gov/pubmed/19940162 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.081380 |
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