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Identification of Transcription Complexes that Contain the Double Bromodomain Protein Brd2 and Chromatin Remodeling Machines

We use affinity purification of the double bromodomain protein Brd2 to isolate a multicomponent nuclear complex from cultured cells, and apply mass spectrometry/proteomics methods to identify the participants. We then confirm by immunoblot several transcription co-activators and co-repressors, prote...

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Detalhes bibliográficos
Main Authors: Denis, Gerald V., McComb, Mark E., Faller, Douglas V., Sinha, Anupama, Romesser, Paul B., Costello, Catherine E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2823066/
https://ncbi.nlm.nih.gov/pubmed/16512664
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr050430u
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