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Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.

Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a single step to amplify a single-copy gene and produce an excess of single-stranded DNA of a chosen strand for d...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Main Authors: Gyllensten, U B, Erlich, H A
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282250/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3174659/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.20.7652
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