Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a single step to amplify a single-copy gene and produce an excess of single-stranded DNA of a chosen strand for d...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1988
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282250/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3174659/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.20.7652 |
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