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Quantification of DNaseI-sensitivity by Real-time PCR: Quantitative Analysis of DNaseI-hypersensitivity of the Mouse β-Globin LCR

We employ real-time PCR to allow us to quantify the sensitivity of chromatin to digestion by DNaseI. This approach has three clear advantages over the more conventional use of the Southern hybridization assay: the accuracy of quantification is improved; the resolution of the assay is enhanced, by de...

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Bibliografiset tiedot
Päätekijät: McArthur, Michael, Gerum, Shawn, Stamatoyannopoulos, George
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2001
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2819987/
https://ncbi.nlm.nih.gov/pubmed/11601844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/jmbi.2001.4969
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