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Quantification of DNaseI-sensitivity by Real-time PCR: Quantitative Analysis of DNaseI-hypersensitivity of the Mouse β-Globin LCR

We employ real-time PCR to allow us to quantify the sensitivity of chromatin to digestion by DNaseI. This approach has three clear advantages over the more conventional use of the Southern hybridization assay: the accuracy of quantification is improved; the resolution of the assay is enhanced, by de...

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Detalhes bibliográficos
Main Authors: McArthur, Michael, Gerum, Shawn, Stamatoyannopoulos, George
Formato: Artigo
Idioma:Inglês
Publicado em: 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2819987/
https://ncbi.nlm.nih.gov/pubmed/11601844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/jmbi.2001.4969
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