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Quantification of DNaseI-sensitivity by Real-time PCR: Quantitative Analysis of DNaseI-hypersensitivity of the Mouse β-Globin LCR
We employ real-time PCR to allow us to quantify the sensitivity of chromatin to digestion by DNaseI. This approach has three clear advantages over the more conventional use of the Southern hybridization assay: the accuracy of quantification is improved; the resolution of the assay is enhanced, by de...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2001
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2819987/ https://ncbi.nlm.nih.gov/pubmed/11601844 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/jmbi.2001.4969 |
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