Properties of Escherichia coli EF-Tu mutants designed for fluorescence resonance energy transfer from tRNA molecules

Here we describe the design, preparation and characterization of 10 EF-Tu mutants of potential utility for the study of Escherichia coli elongation factor Tu (EF-Tu) interaction with tRNA by a fluorescence resonance energy transfer assay. Each mutant contains a single cysteine residue at positions i...

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Detalhes bibliográficos
Main Authors: Perla-Kajan, Joanna, Lin, Xin, Cooperman, Barry S., Goldman, Emanuel, Jakubowski, Hieronim, Knudsen, Charlotte R., Mandecki, Wlodek
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2010
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Original articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2816605/
https://ncbi.nlm.nih.gov/pubmed/20083494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzp079
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