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Targeted Integration of T-DNA into the Tobacco Genome at Double-Stranded Breaks: New Insights on the Mechanism of T-DNA Integration

Agrobacterium tumefaciens T-DNA normally integrates into random sites in the plant genome. We have investigated targeting of T-DNA by nonhomologous end joining process to a specific double-stranded break created in the plant genome by I-CeuI endonuclease. Sequencing of genomic DNA/T-DNA junctions in...

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Autori principali: Chilton, Mary-Dell M., Que, Qiudeng
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Plant Biologists 2003
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC281593/
https://ncbi.nlm.nih.gov/pubmed/14551336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.103.026104
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