Expression, purification and X-ray analysis of 1,3-­propanediol dehydrogenase (Aq_1145) from Aquifex aeolicus VF5

1,3-Propanediol dehydrogenase is an enzyme that catalyzes the oxidation of 1,3-­propanediol to 3-hydroxypropanal with the simultaneous reduction of NADP(+) to NADPH. SeMet-labelled 1,3-propanediol dehydrogenase protein from the hyperthermophilic bacterium Aquifex aeolicus VF5 was overexpressed in Es...

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Detalhes bibliográficos
Main Authors: Jeyakanthan, Jeyaraman, Thamotharan, Subbiah, Panjikar, Santosh, Kitamura, Yoshiaki, Nakagawa, Noriko, Shinkai, Akeo, Kuramitsu, Seiki, Yokoyama, Shigeyuki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2010
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2815688/
https://ncbi.nlm.nih.gov/pubmed/20124718
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109052403
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