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Preparation and characterization of (15)N-enriched, size-defined heparan sulfate precursor oligosaccharides

We report the preparation of size-defined [(15)N]N-acetylheparosan oligosaccharides from Escherichia coli-derived (15)N-enriched N-acetylheparosan. Optimized growth conditions of E. coli in minimal media containing (15)NH(4)Cl yielded [(15)N]N-acetylheparosan on a preparative scale. Depolymerization...

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Bibliografiske detaljer
Main Authors: Sigulinsky, Crystal, Babu, Ponnusamy, Victor, Xylophone V., Kuberan, Balagurunathan
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2812664/
https://ncbi.nlm.nih.gov/pubmed/19945695
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.carres.2009.10.024
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