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The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing

BACKGROUND: We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is present at high levels in embryonic stem cells and Purkinje neurons. The methylation status of cytosines is typically assessed by reaction with sodium bisulfite followed by...

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Autori principali: Huang, Yun, Pastor, William A., Shen, Yinghua, Tahiliani, Mamta, Liu, David R., Rao, Anjana
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2010
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2811190/
https://ncbi.nlm.nih.gov/pubmed/20126651
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0008888
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