LC/MS/MS Analysis of N-Terminal Protein Adducts with Improved Sensitivity: A Comparison of Selected Edman Isothiocyanate Reagents

This study provides a basis for a new and straightforward method for LC/MS/MS-based screening of N-terminal protein adducts. This procedure is denoted the “FIRE procedure” as fluorescein isothiocyanate (FITC) gave superior sensitivity by LC/MS/MS when measuring adducts (R) of electrophilic compounds...

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Detalhes bibliográficos
Main Authors: Rydberg, Per, von Stedingk, Hans, Magnér, Jörgen, Björklund, Jonas
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2009
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2809355/
https://ncbi.nlm.nih.gov/pubmed/20107558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2009/153472
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