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Free-Solution, Label-Free Protein-Protein Interactions Characterized by Dynamic Light Scattering
We report a free-solution, label-free method for quantitative characterization of macromolecular interactions using dynamic light scattering, a temperature controlled plate reader, and a multiwell concentration gradient. This nondestructive technique enabled determination of stoichiometry of binding...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2808485/ https://ncbi.nlm.nih.gov/pubmed/20338851 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.09.061 |
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