Free-Solution, Label-Free Protein-Protein Interactions Characterized by Dynamic Light Scattering

We report a free-solution, label-free method for quantitative characterization of macromolecular interactions using dynamic light scattering, a temperature controlled plate reader, and a multiwell concentration gradient. This nondestructive technique enabled determination of stoichiometry of binding...

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Bibliografske podrobnosti
Main Authors: Hanlon, Amy D., Larkin, Michael I., Reddick, Ryan M.
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2010
Teme:
Protein
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2808485/
https://ncbi.nlm.nih.gov/pubmed/20338851
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2009.09.061
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