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Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

BACKGROUND: The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting sufficient signal for image analysis. We describe here...

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Bibliografiska uppgifter
Huvudupphovsmän: Spiess, Andrej-Nikolai, Mueller, Nadine, Ivell, Richard
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2003
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC280674/
https://ncbi.nlm.nih.gov/pubmed/14606961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-44
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