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Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

BACKGROUND: The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting sufficient signal for image analysis. We describe here...

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書誌詳細
主要な著者: Spiess, Andrej-Nikolai, Mueller, Nadine, Ivell, Richard
フォーマット: Artigo
言語:Inglês
出版事項: BioMed Central 2003
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC280674/
https://ncbi.nlm.nih.gov/pubmed/14606961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-44
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