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Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations
BACKGROUND: The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting sufficient signal for image analysis. We describe here...
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| 主要な著者: | , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2003
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| 主題: | |
| オンライン・アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC280674/ https://ncbi.nlm.nih.gov/pubmed/14606961 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-44 |
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