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Amplified RNA degradation in T7-amplification methods results in biased microarray hybridizations

BACKGROUND: The amplification of RNA with the T7-System is a widely used technique for obtaining increased amounts of RNA starting from limited material. The amplified RNA (aRNA) can subsequently be used for microarray hybridizations, warranting sufficient signal for image analysis. We describe here...

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Detalhes bibliográficos
Main Authors: Spiess, Andrej-Nikolai, Mueller, Nadine, Ivell, Richard
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2003
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC280674/
https://ncbi.nlm.nih.gov/pubmed/14606961
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-4-44
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