Recombinant protein expression by targeting pre-selected chromosomal loci

BACKGROUND: Recombinant protein expression in mammalian cells is mostly achieved by stable integration of transgenes into the chromosomal DNA of established cell lines. The chromosomal surroundings have strong influences on the expression of transgenes. The exploitation of defined loci by targeting...

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Main Authors: Nehlsen, Kristina, Schucht, Roland, da Gama-Norton, Leonor, Krömer, Wolfgang, Baer, Alexandra, Cayli, Aziz, Hauser, Hansjörg, Wirth, Dagmar
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2009
Fag:
Research article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2804664/
https://ncbi.nlm.nih.gov/pubmed/20003421
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-9-100
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