Determination of the Dissociation Constants for Ca(2+) and Calmodulin from the Plasma Membrane Ca(2+) Pump by a Lipid Probe That Senses Membrane Domain Changes

The purpose of this work was to obtain information about conformational changes of the plasma membrane Ca(2+)-pump (PMCA) in the membrane region upon interaction with Ca(2+), calmodulin (CaM) and acidic phospholipids. To this end, we have quantified labeling of PMCA with the photoactivatable phospha...

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Bibliographic Details
Main Authors: Mangialavori, Irene, Ferreira-Gomes, Mariela, Pignataro, María F., Strehler, Emanuel E., Rossi, Juan Pablo F. C.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2010
Subjects:
Membrane Transport, Structure, Function, and Biogenesis
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2804156/
https://ncbi.nlm.nih.gov/pubmed/19892708
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.076679
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