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Determination of the Dissociation Constants for Ca(2+) and Calmodulin from the Plasma Membrane Ca(2+) Pump by a Lipid Probe That Senses Membrane Domain Changes
The purpose of this work was to obtain information about conformational changes of the plasma membrane Ca(2+)-pump (PMCA) in the membrane region upon interaction with Ca(2+), calmodulin (CaM) and acidic phospholipids. To this end, we have quantified labeling of PMCA with the photoactivatable phospha...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Biochemistry and Molecular Biology
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2804156/ https://ncbi.nlm.nih.gov/pubmed/19892708 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.076679 |
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