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DNA Polymerase Fidelity: Comparing Direct Competition of Right and Wrong dNTP Substrates with Steady State and Presteady State Kinetics

DNA polymerase fidelity is defined as the ratio of right (R) to wrong (W) nucleotide incorporations when dRTP and dWTP substrates compete at equal concentrations for primer extension at the same site in the polymerase-primer-template DNA complex. Typically, R incorporation is favored over W by 10(3)...

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Detalhes bibliográficos
Main Authors: Bertram, Jeffrey G., Oertell, Keriann, Petruska, John, Goodman, Myron F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2803633/
https://ncbi.nlm.nih.gov/pubmed/20000359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901653g
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