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Comparison of Four Nuclear Isolation Buffers for Plant DNA Flow Cytometry
• Background and Aims DNA flow cytometry requires preparation of suspensions of intact nuclei, which are stained using a DNA-specific fluorochrome prior to analysis. Various buffer formulas were developed to preserve nuclear integrity, protect DNA from degradation and facilitate its stoichiometric s...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2006
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2803574/ https://ncbi.nlm.nih.gov/pubmed/16820407 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/aob/mcl141 |
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